Composite

Part:BBa_K1124100

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP, +UV reset device(E234K, -LVA)

The full construct for Multicellular Analog Clock. 

 LuxI and LuxR forms the positive feedback loop of AHL production.
 TetR, AiiA, and sRNAs are the suppressors for negative feedback loops.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).
 amilGFP is the yellow reporter protein expressed constitutively as an internal standard.
 cI repressor and PR promoter forms UV reset device to make refractory period with UV induction.

You can make the Multicellular Analog Clock with this BioBrick part (BBa_K1124100). The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6228
    Illegal NheI site found at 6251
    Illegal NheI site found at 7215
    Illegal NheI site found at 7238
    Illegal NheI site found at 8159
    Illegal NheI site found at 8182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 885
    Illegal AgeI site found at 5361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2353
    Illegal BsaI.rc site found at 2681
    Illegal BsaI.rc site found at 3009


[edit]
Categories
Parameters
None